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commercial fluorometer  (Promega)

 
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    Promega commercial fluorometer
    Commercial Fluorometer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/commercial fluorometer/product/Promega
    Average 90 stars, based on 1 article reviews
    commercial fluorometer - by Bioz Stars, 2026-06
    90/100 stars

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    Schematic of the complete device used in this study is presented. The device comprises a fluorometer with an integrated graphene oxide (GO)–aptamer biosensor and a custom smartphone application. ( a ) The fluorometer is combined with the smartphone application. ( b ) An illustration of the GO–aptamer assay principle demonstrates that the fluorescence signal is absent when fluorescence-labeled aptamers bind to GO, resulting in fluorescence quenching. In the presence of the target molecules, the aptamers bind to them, dissociating from GO and leading to the recovery of the fluorescence signal.

    Journal: Biosensors

    Article Title: Development of a High-Accuracy, Low-Cost, and Portable Fluorometer with Smartphone Application for the Detection of Urinary Albumin towards the Early Screening of Chronic Kidney and Renal Diseases

    doi: 10.3390/bios13090876

    Figure Lengend Snippet: Schematic of the complete device used in this study is presented. The device comprises a fluorometer with an integrated graphene oxide (GO)–aptamer biosensor and a custom smartphone application. ( a ) The fluorometer is combined with the smartphone application. ( b ) An illustration of the GO–aptamer assay principle demonstrates that the fluorescence signal is absent when fluorescence-labeled aptamers bind to GO, resulting in fluorescence quenching. In the presence of the target molecules, the aptamers bind to them, dissociating from GO and leading to the recovery of the fluorescence signal.

    Article Snippet: These results were then compared with those obtained using a commercial fluorometer (Quantus TM , Promega).

    Techniques: Fluorescence, Labeling

    Photographs illustrating the developed portable fluorometer. ( a ) The complete body of the portable fluorometer. ( b ) The front side, showing the connection between the circuit board and the fluorescence chamber. ( c ) The back side of the circuit board, which does not include any devices to support one-sided pick-and-place assembly.

    Journal: Biosensors

    Article Title: Development of a High-Accuracy, Low-Cost, and Portable Fluorometer with Smartphone Application for the Detection of Urinary Albumin towards the Early Screening of Chronic Kidney and Renal Diseases

    doi: 10.3390/bios13090876

    Figure Lengend Snippet: Photographs illustrating the developed portable fluorometer. ( a ) The complete body of the portable fluorometer. ( b ) The front side, showing the connection between the circuit board and the fluorescence chamber. ( c ) The back side of the circuit board, which does not include any devices to support one-sided pick-and-place assembly.

    Article Snippet: These results were then compared with those obtained using a commercial fluorometer (Quantus TM , Promega).

    Techniques: Fluorescence

    Simplified circuit diagram of the fluorometer.

    Journal: Biosensors

    Article Title: Development of a High-Accuracy, Low-Cost, and Portable Fluorometer with Smartphone Application for the Detection of Urinary Albumin towards the Early Screening of Chronic Kidney and Renal Diseases

    doi: 10.3390/bios13090876

    Figure Lengend Snippet: Simplified circuit diagram of the fluorometer.

    Article Snippet: These results were then compared with those obtained using a commercial fluorometer (Quantus TM , Promega).

    Techniques:

    The mobile application used with the fluorometer. ( a ) The software’s welcome screen. ( b ) Options for the user to choose between glycated albumin and HSA. ( c ) An example of instructions guiding users to properly prepare a sample. ( d ) An example of the incubation process during the preparation of a sample. ( e ) The last step of sample preparation, with an error showing that the application is not connected to the machine. ( f ) An example screen when the application receives intensity data from the machine.

    Journal: Biosensors

    Article Title: Development of a High-Accuracy, Low-Cost, and Portable Fluorometer with Smartphone Application for the Detection of Urinary Albumin towards the Early Screening of Chronic Kidney and Renal Diseases

    doi: 10.3390/bios13090876

    Figure Lengend Snippet: The mobile application used with the fluorometer. ( a ) The software’s welcome screen. ( b ) Options for the user to choose between glycated albumin and HSA. ( c ) An example of instructions guiding users to properly prepare a sample. ( d ) An example of the incubation process during the preparation of a sample. ( e ) The last step of sample preparation, with an error showing that the application is not connected to the machine. ( f ) An example screen when the application receives intensity data from the machine.

    Article Snippet: These results were then compared with those obtained using a commercial fluorometer (Quantus TM , Promega).

    Techniques: Incubation, Sample Prep

    Calibration curves for HSA detection using an aptasensor with two different fluorometers: ( a ) the Quantus TM commercial fluorometer and ( b ) the developed fluorometer. The curves plot fluorescence response against HSA concentration. Both devices exhibited sigmoidal correlation within the 0–1.6 mg/mL range. The Quantus TM commercial fluorometer results show two linear correlations, one between 0 and 200 μg/mL (upper graph ( a )) with Y = 0.0006593(X) + 0.01042 (R 2 = 0.98485) and another between 200 and 1600 μg/mL (lower graph ( a )) with Y = 0.02442(X) − 6.34609 (R 2 = 0.99374). The developed fluorometer results also display two linear correlations, one within 0–200 μg/mL (upper graph ( b )) with Y = 0.0017(X) + 0.0213 (R 2 = 0.93056) and another between 200 and 1600 μg/mL (lower graph ( b )) with Y = 0.5557(X) − 12.98087 (R 2 = 0.9961).

    Journal: Biosensors

    Article Title: Development of a High-Accuracy, Low-Cost, and Portable Fluorometer with Smartphone Application for the Detection of Urinary Albumin towards the Early Screening of Chronic Kidney and Renal Diseases

    doi: 10.3390/bios13090876

    Figure Lengend Snippet: Calibration curves for HSA detection using an aptasensor with two different fluorometers: ( a ) the Quantus TM commercial fluorometer and ( b ) the developed fluorometer. The curves plot fluorescence response against HSA concentration. Both devices exhibited sigmoidal correlation within the 0–1.6 mg/mL range. The Quantus TM commercial fluorometer results show two linear correlations, one between 0 and 200 μg/mL (upper graph ( a )) with Y = 0.0006593(X) + 0.01042 (R 2 = 0.98485) and another between 200 and 1600 μg/mL (lower graph ( a )) with Y = 0.02442(X) − 6.34609 (R 2 = 0.99374). The developed fluorometer results also display two linear correlations, one within 0–200 μg/mL (upper graph ( b )) with Y = 0.0017(X) + 0.0213 (R 2 = 0.93056) and another between 200 and 1600 μg/mL (lower graph ( b )) with Y = 0.5557(X) − 12.98087 (R 2 = 0.9961).

    Article Snippet: These results were then compared with those obtained using a commercial fluorometer (Quantus TM , Promega).

    Techniques: Fluorescence, Concentration Assay

    Correlation between the fluorescence intensities measured by the developed fluorometer and the commercial fluorometer in the analysis of albumin concentration in 130 urine samples (black dots). The linear equation for the correlation is y = 0.92628x − 16.134, and the coefficient of determination (R 2 ) is 0.9652, indicating a strong positive relationship between the measurements.

    Journal: Biosensors

    Article Title: Development of a High-Accuracy, Low-Cost, and Portable Fluorometer with Smartphone Application for the Detection of Urinary Albumin towards the Early Screening of Chronic Kidney and Renal Diseases

    doi: 10.3390/bios13090876

    Figure Lengend Snippet: Correlation between the fluorescence intensities measured by the developed fluorometer and the commercial fluorometer in the analysis of albumin concentration in 130 urine samples (black dots). The linear equation for the correlation is y = 0.92628x − 16.134, and the coefficient of determination (R 2 ) is 0.9652, indicating a strong positive relationship between the measurements.

    Article Snippet: These results were then compared with those obtained using a commercial fluorometer (Quantus TM , Promega).

    Techniques: Fluorescence, Concentration Assay

    Correlation analysis of measurements obtained using the developed fluorometer, the commercial fluorometer, and the standard method. ( a ) Comparison between the developed fluorometer and the commercial fluorometer, revealing a linear relationship described by the equation y = 0.81496x – 0.00144, with an R 2 value of 0.98095. ( b ) Relationship between the commercial fluorometer and the standard method, characterized by the equation y = 1.26442x – 0.06416 and an R 2 value of 0.96806. ( c ) Correlation between the developed fluorometer and the standard method, with a linear equation of y = 1.48508x − 0.06243 and an R 2 value of 0.9446.

    Journal: Biosensors

    Article Title: Development of a High-Accuracy, Low-Cost, and Portable Fluorometer with Smartphone Application for the Detection of Urinary Albumin towards the Early Screening of Chronic Kidney and Renal Diseases

    doi: 10.3390/bios13090876

    Figure Lengend Snippet: Correlation analysis of measurements obtained using the developed fluorometer, the commercial fluorometer, and the standard method. ( a ) Comparison between the developed fluorometer and the commercial fluorometer, revealing a linear relationship described by the equation y = 0.81496x – 0.00144, with an R 2 value of 0.98095. ( b ) Relationship between the commercial fluorometer and the standard method, characterized by the equation y = 1.26442x – 0.06416 and an R 2 value of 0.96806. ( c ) Correlation between the developed fluorometer and the standard method, with a linear equation of y = 1.48508x − 0.06243 and an R 2 value of 0.9446.

    Article Snippet: These results were then compared with those obtained using a commercial fluorometer (Quantus TM , Promega).

    Techniques: Comparison